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mouse anti isl1 2 antibody  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank mouse anti isl1 2 antibody
    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number <t>of</t> <t>ISL1/2</t> + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
    Mouse Anti Isl1 2 Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+isl1+2/anti-Islet-1+%26+Islet-2+homeobox/bio_rxiv__64898__2026__05__07__723626-324-50-54
    Average 96 stars, based on 279 article reviews
    mouse anti isl1 2 antibody - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Engineering a pacemaker-driven human mini-heart guided by spatial and single cell multi-omics of sinoatrial node development"

    Article Title: Engineering a pacemaker-driven human mini-heart guided by spatial and single cell multi-omics of sinoatrial node development

    Journal: bioRxiv

    doi: 10.64898/2026.05.07.723626

    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number of ISL1/2 + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
    Figure Legend Snippet: a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number of ISL1/2 + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.

    Techniques Used: Fluorescence, Control, Staining

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    Article Snippet: Mouse anti Isl1/2 , Developmental Studies Hybridoma bank , Cat#39.4DS.

    Article Title: Reduced Availability of Essential Amino Acids Disrupts Differentiation of Anorexigenic POMC Neurons in the Fetal Rat Hypothalamus
    Article Snippet: The procedure was performed as described before [ ], using the RNAscope Multiplex Fluorescent Reagent Kit v2 (#323100, ACD Technologies), the HybEZ II Oven (40 °C), and the custom Rn- Npy -O1-C1 Probe (#1211911-C1, ACD Technologies) for detection of Rattus norvegicus Npy mRNA.

    Cell-Signaling:

    Article Title: Spinal motor neuron development and metabolism are transcriptionally regulated by nuclear factor IA
    Article Snippet: .. Primary antibodies used were as follows: rat anti-BrdU (Abcam; 1:500), rabbit anti-Caspase3 (R&D Biosystems; 1:500), mouse anti-CGRP (CBT; 1:150), mouse anti-Chx10 (SCBT; 1:50), rabbit anti-ER81 [1:30,000 ( )], goat anti-Foxp1 (R&D Biosystems; 1:100), rabbit anti-Foxp1 (Sigma-Aldrich; 1:100), rabbit anti-Foxp2 (Abcam; 1:2000), rabbit anti-GFP (Genetex, 1:700), rabbit anti-Isl1 (Abcam; 1:400), mouse anti-Isl1 (DSHB; 1:25), mouse anti-Isl1/2 (DSHB; 1:50), rabbit anti-Ki67 (Cell Signaling; 1:500), mouse anti-Lhx1/5 (DSHB; 1:5), rabbit anti-Lhx3 (Abcam; 1:400), mouse anti-Mnr2 (DSHB; 1:10), mouse anti-NF (DSHB; 1:70), rabbit-anti Nfia (Sigma-Aldrich; 1:500), rabbit anti-Nfib (Sigma-Aldrich; 1:300), mouse anti-Nkx2.2 (DSHB; 1:5), mouse anti-Nkx6.1 (DSHB; 1:10), guinea pig anti-Nkx6.2 [1:700; ( )], rabbit anti-Olig2 (Sigma-Aldrich; 1:250), rabbit anti-Pax2 (Thermo Fisher Scientific; 1:100), and rabbit anti-Sox9 (Millipore; 1:800). ..

    Article Title: Lhx4 surpasses its paralog Lhx3 in promoting the differentiation of spinal V2a interneurons
    Article Snippet: .. Primary antibodies against the following proteins were used: sheep anti-Chx10 (Exalpha Biologicals #X1179P) at 1:200, anti-Cleaved Caspase-3 (Cell signaling #ASP 175) at 1:100, goat anti-Foxp1 (R&D #AF4534) at 1:500, rat anti-Gata3 (Absea Biotechnology #111214D02) at 1:15 or rabbit anti-Gata3 (Cell signaling #5852) at 1:200, chicken anti-GFP (Aves lab #GFP-1020) at 1:2000, mouse anti-Hb9/Mnr2 (DSHB #81.5C10) at 1:2000, goat anti-Isl1/2 (Neuromics #GT15051) at 1:1000 or mouse anti-Isl1/2 (DSHB #39.4D5) at 1:6000 or rabbit anti-Isl1 (Abcam #ab26122) at 1:1000, guinea pig anti-Lhx3 [ ] at 1:250, rabbit anti-Lhx4 (Proteintech #11183-1-AP) at 1:300, guinea pig anti-MafA [ ] at 1:500, rabbit anti-MafA (Novus Biological #NB400-137) at 1:500, guinea pig anti-OC1/HNF6 [ ] at 1:6000, sheep anti-OC1/HNF6 (R&D # AF6277) at 1:250, rat anti-OC2 [ ], rabbit anti-Olig2 (Millipore #AB9610) at 1:4000, mouse anti-Shox2 (Abcam #ab55740) at 1:200 and guinea-pig anti-Sox14 [ ] at 1:1000. .. Following secondary antibodies were used: donkey anti-goat/AlexaFluor 594, donkey anti-mouse/AlexaFluor 594, goat anti-mouse IgG1/AlexaFluor 594, donkey anti-rabbit/AlexaFluor 594, donkey anti-rat/AlexaFluor 594, donkey anti-guinea pig/AlexaFluor594, donkey anti-rabbit/AlexaFluor 647, donkey anti-rat/AlexaFluor647, donkey anti-rabbit/AlexaFluor488, donkey anti-goat/AlexaFluor488, donkey anti-rat/AlexaFluor488, donkey anti-mouse/AlexaFluor488, donkey anti-chicken/AlexaFluor 488 and donkey anti-guinea pig/AlexaFluor488 purchased from ThermoFisher Scientific or Jackson Laboratories and used at dilution 1:500.



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    Developmental Studies Hybridoma Bank mouse anti isl1 2 antibody
    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number <t>of</t> <t>ISL1/2</t> + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
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    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number <t>of</t> <t>ISL1/2</t> + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
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    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number <t>of</t> <t>ISL1/2</t> + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
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    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number <t>of</t> <t>ISL1/2</t> + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
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    Image Search Results


    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number of ISL1/2 + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.

    Journal: bioRxiv

    Article Title: Engineering a pacemaker-driven human mini-heart guided by spatial and single cell multi-omics of sinoatrial node development

    doi: 10.64898/2026.05.07.723626

    Figure Lengend Snippet: a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number of ISL1/2 + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.

    Article Snippet: At 54 hpf larvae were fixed in 2% PFA for 2 hours at room temperature, washed extensively in PBS with 0.3% TritonX-100 (PBX), incubated in blocking buffer (BB: PBX, 0.5% BSA, 10% goat serum) for 2 hours at room temperature, incubated overnight at 4°C in BB containing 1/50 dilution of mouse anti-ISL1/2 antibody (Iowa Developmental Studies Hybridoma Bank #39.4D5), washed extensively with PBX, incubated at room temperature for 2 hours in BB with a 1/200 dilution of goat anti-mouse IgG2b Alexa Fluor568 (Invitrogen #A-21144), washed extensively with PBX, and mounted in 1:1 PBS:glycerol for imaging on a Zeiss LSM800 confocal microscope.

    Techniques: Fluorescence, Control, Staining